@Article{LimaPiNoRiCaBaMo:2016:InVaAl,
author = "Lima, Domenica Teixeira de and Piedras, Fernanda Reinhardt and
Nogueira, Fl{\'a}via Previero and Richard, Eduardo da Cunha and
Castaldi Tocci, Bianca Reis and Barrera Alba, Jose Juan and Moser,
Gleyci Aparecida Oliveira",
affiliation = "{Universidade do Estado do Rio de Janeiro (UERJ)} and
{Universidade do Estado do Rio de Janeiro (UERJ)} and
{Universidade Federal de S{\~a}o Paulo (UNIFESP)} and
{Universidade do Estado do Rio de Janeiro (UERJ)} and
{Universidade do Estado do Rio de Janeiro (UERJ)} and {Instituto
Nacional de Pesquisas Espaciais (INPE)} and {Universidade do
Estado do Rio de Janeiro (UERJ)}",
title = "Intraspecific variation in alkaline phosphatase activity in
Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)",
journal = "Acta Botanica Brasilica",
year = "2016",
volume = "30",
number = "3",
pages = "445--454",
month = "Juy/Sept.",
keywords = "alkaline phosphatase, auxotrophy, marine diatom, organic
phosphorus incorporation, phosphate limitation.",
abstract = "To describe potential intraspecific variation in phosphorus
incorporation in two strains of Phaeodactylum tricornutum
(Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was
evaluated via enzyme-labeled fluorescence assay. Analysis using
the probe ELF-97 (R) provides individual evaluation, and therefore
can determine the nutritional status of inorganic phosphorus in
phytoplanktonic cells. Bioassays compared the control treatment to
both phosphate-enriched and phosphate-depleted treatments by
varying only the phosphate concentration in the media. The P.
tricornutum strains exhibited differences in their development
when incubated in the phosphate-enriched media. The development of
the Ub7 strain differed by exhibiting {"}luxury uptake{"} and
utilization of organic phosphorus, and the alkaline phosphatase
analysis indicated limitations of this clone under such
conditions. The Ub7 strain showed higher AP activity, when
compared to Ub3, in the P-enriched condition. P. tricornutum
presented increases in AP activity and low variation in
Surface/Volume ratio, by increasing biovolume and its maximum
linear dimension, as strategies for phosphate incorporation. Our
results highlight intraspecific differences in alkaline
phosphatase activity, and hence differences in the incorporation
of organic phosphorus, as the tested species regulated enzymatic
activity under different external phosphate concentrations.",
doi = "10.1590/0102-33062016abb0149",
url = "http://dx.doi.org/10.1590/0102-33062016abb0149",
issn = "0102-3306",
language = "en",
targetfile = "Lima_intra.pdf",
urlaccessdate = "27 abr. 2024"
}